The paper introduces a kind of virtual signal analysis instrument which is based on VXIbus hardware and graphical programming language HPVEE. The procedure and the method of designing the virtual instrument are specified,and the function of the instrument are presented in detail. The hardware is configured simply and the Chinese soft panel is friendly. The virtual instrument not only has the function of a signal analysis instrument, but also can realize the measurement of signal's frequency, period, pulse width, rise time and fall time.